Foxy Fraulien gave Singleton her first winner as a trainer when she won her maiden at the Gold Coast in July last year.
The five-year-old has since gone on to win a further three times including a 1500 metre Class 2 event at Kilcoy in March.
It was her only Kilcoy appearance when she led all the way for apprentice Cody Collis.
This time Singleton has booked her partner, Wanderson D'Avila, in the Benchmark 60 Handicap over 1500 metres.
Foxy Fraulien’s recent form has been mixed, beating one home at Gatton over 1400 metres, before a close second in a 1710 metre Class 4 race at Ipswich on September 25.